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Multiple Persistent Environmental Chemicals at Low Concentrations Differentially Regulates Fatty Acids/Lipids in Wild Type and PPARAa−/− Mice
Author(s) -
Gonnabathula Pavani K,
Yakubu Momoh A
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb25
Subject(s) - chemistry , arachidonic acid , fatty acid , kidney , pentadecanoic acid , eicosapentaenoic acid , medicine , docosahexaenoic acid , endocrinology , polyunsaturated fatty acid , biochemistry , food science , biology , enzyme
Many chemicals are detected in the environment at very low levels, they were viewed as being incapable of causing harm. Their risks to human health and associated environment are now emerging. But effects of their multiple interactions at cellular and molecular levels is unknown. We have investigated the effects of treatment of wild type and PPARα knockout mice on fatty acid contents in different organs. Mice were treated with low concentrations of multiple persistent environmental chemicals: atrazine, dieldrin, endrin, endosulfan and anthracene (1–100ng/L) in drinking water for six weeks and brain, liver, kidney, heart and spleen were collected, processed for fatty acid/lipid contents determination by GC/MS. The percentage changes in the levels of the fatty acid following treatment were calculated for wild type (WT) and PPARα −/− (KO). The changes (WT and KO) were compared to find the effects of treatment and the genetic (PPARα) influence on the regulation of fatty acid levels. Our results show differential presence of fatty acids: hexadecanoic acid, hexadecanoic acid methyl ester, hexadecanamide, 9‐octadecanemaide, pentadecanoic acid, octadecanoid acid, arachidonic acid, and cholesterol in the different organs as well as their differential regulation in the WT and KO. Results are summarized in Tables 1 and 2. Briefly, treatment increased 9‐octadecanemaide by 145% (WT) and 38% (KO) in the heart, reduced in the kidney 17% (WT), 29% (KO); hexadecanoic acid, octadecanoic acid was increased 0% (WT) but by 178% and 110% respectively (KO) (kidney), octadecanoic acid went up by 334% (KO) with not change in WT, hexadecanamide increased by 92% in KO Liver with 5% in WT. Generally, more perturbations in the regulation of the levels of fatty acid species in the KO than in the WT. These results indicate that multiple persistent environmental chemicals at low concentrations have limited effects on fatty acid dynamics in the WT but enhanced possible dysregulations in the PPARAα−/− mice. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .