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Three‐dimensional reconstruction of Prdm family gene expression patterns during mouse development stage by tissue clearing technique
Author(s) -
Woo Jiwon,
Kang Haewon,
Cho YongEun
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb189
Subject(s) - biology , gene , embryo , gene expression , microbiology and biotechnology , embryonic stem cell , regulation of gene expression , embryogenesis , genetics
The Prdm family has recently spawned considerable interest as it has been implicated in fundamental aspects of cellular differentiation and exhibits expanding ties to human diseases. Several Prdm family members are deregulated in human diseases, most prominently in solid cancers, where they can act as both tumor suppressors or drivers of oncogenic processes. Some Prdm genes might play an essential role in gene expression were confined to the mammalian central nervous system. We hypothesized that the Prdm gene family may also significantly participate in the development of the mammalian nervous system is required to explore how the gene by the advanced pathological analysis. To assess Prdm family gene expressions patterns at the formation of central nervous system during mouse development stage. The study of the genetic regulation of embryonic development requires the three‐dimensional mapping of gene expression at the high‐resolution microscopic level. We generated transparent mouse embryos by new modified passive tissue clearing technique called mPACT method. Mouse embryos of sequential stage were obtained from three distinct developmental stages, intact embryos was optical transparency to mPACT methods in few days. We reconstructed three‐dimensional expression patterns of Prdm family and blood vessel using transparent mouse embryos. Partial Prdm gene expression was concentrated in the brain and spinal cord regions of each embryo. In this study, we developed embryo‐specific simple clearing method for creating see‐through mouse embryo complete with transparent organ. In addition, our study reconfirmed that some Prdm gene might be a transcriptional regulator for normal neural tissue differentiation during mouse embryonic development using three‐dimensional analysis and transparent embryos. Support or Funding Information This work was supported by the Brain Korea 21 PLUS Project for Medical Science, Yonsei University. In addition, this work was supported by a grant from the National Research Foundation of Korea (NRF‐2017R1D1A1B03030315). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .