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Osteocyte morphology in the human craniofacial skeleton
Author(s) -
Guerrero Kristi,
Dechow Paul C,
McGeeLawrence Meghan,
Yu Kanglun,
McIntosh Leslie Pryor
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb153
Subject(s) - osteocyte , craniofacial , anatomy , condyle , biology , osteoblast , biochemistry , genetics , in vitro
Osteocyte size and shape has been shown to vary by anatomical region, variation which reflects differences in osteocyte sensitivity to strain. Variation in osteocyte morphology should therefore be considered when interpreting primate craniofacial adaptations. Very little is known about how osteocyte shape and size vary in the human craniofacial skeleton. For this study, osteocyte size and shape in the human mandibular condyle was analyzed and compared to osteocyte size and shape in the human supraorbital and zygomatic regions from 2 individuals. A multiphoton confocal microscope was used to collect 3‐dimensional images from FITC stained human mandibular condyles and compared to existing data for the supraorbital and zygomatic regions from 2 individuals. Imaris analysis of osteocyte cell bodies show that osteocytes in the supraorbital region are significantly larger than in the zygoma and condyle (p≤0.001). Shape of osteocytes was measured and analyzed by creating a bounding box around 15–20 osteonal osteocytes per individual and comparing ellipsoid axis lengths a, b, and c. ANOVA testing found that osteocyte shape variation was non‐significant between the three locations. Osteocytes in all three cranial locations are mostly oblate in shape (disc‐like; where c>a). These preliminary results suggest that osteocyte morphology may be similar across the craniofacial skeleton; differences in osteocyte size may indicate metabolic differences across craniofacial regions. Support or Funding Information NSF BCS 0725126 NSF CMMI 1727949 Gail Dillard Faculty Enrichment Fund, ABAC FoundationThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .