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Development of a Targeted Diversifier Allowing Mutation of All Nucleotide Types In Vivo
Author(s) -
Dueber John,
Halperin Shakked,
Schaffer David
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.95.2
Subject(s) - computational biology , biology , genomics , mutagenesis , genome , genetics , computer science , mutation , gene
The capacity to diversify genetic codes advances our understanding and engineering of biological systems. A method to continuously diversify user‐defined regions of a genome without requiring the integration of nucleic acid libraries would enable forward genetic approaches in systems not amenable to high efficiency homology‐directed integration, rapid evolution of biotechnologically useful activity through accelerated and parallelized rounds of mutagenesis and selection, and cell lineage tracking. Here we developed EvolvR, the first system that can continuously diversify all nucleotides within a tunable window length at user‐defined loci. Our results demonstrate that EvolvR enables multiplexed and continuous diversification of user‐defined genomic loci that will be useful for a broad range of basic and biotechnological applications. Support or Funding Information Innovative Genomics Institute This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .