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Anti‐inflammatory Effect of Table Grapes in Apc(Min/+) Mouse Model of Intestinal Tumorigenesis
Author(s) -
Indukuri Vijaya,
Mason Madeline,
Reddivari Lavanya,
Vanamala Jairam,
Lambert Joshua
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.872.2
Subject(s) - inflammation , ileum , carcinogenesis , lipopolysaccharide , medicine , cytokine , immunology , interleukin 6 , endocrinology , cancer , biology
The link between inflammation and cancer is well‐studied. Epidemiological and experimental data suggests that chronic inflammation plays a very important role in the promotion and progression of colon cancer, and that diet can play an important role in mitigation of chronic inflammation. Previously, our laboratory has found that freeze‐dried whole table grape powder (FDGP) can reduce tumorigenesis in APC(Min/+) mice. It has been reported that inflammation plays an important role in tumorigenesis in this model. The objective of the current study is to assess the anti‐inflammatory activity of FDGP in the Apc(Min/+) mouse model. Male Apc(Min/+) mice (4 wks, n= 8/group) were fed diets supplemented with 3% or 6 % FDGP or control diet for 12 wks. At the end of dietary intervention, expression of inflammation‐related markers in the ileum and the plasma were measured by qRT‐PCR and multiplex ELISA, respectively. FDGP supplementation suppressed the levels of the pro‐inflammatory cytokines IL‐1β, IL‐6, IL‐12, IL‐17 (by 30–70%), and enhanced the levels of the anti‐inflammatory cytokine IL‐10 (by ~50%) in both the ileum and serum compared to control‐fed animals. FDGP‐ fed animals also had significantly lower plasma lipopolysaccharide levels (3–5 fold) and reduced mRNA expression of Claudin 2 (~2 fold) in the ileum, suggesting that FDGP improved gut barrier function compared to the control diet. Overall, our results show the potential anti‐inflammatory effect of table grapes in an animal model of intestinal tumorigenesis and indicate the need for further preclinical mechanistic studies. Support or Funding Information California Table Grape CommissionDepartment of Food Science, Pennsylvania State University This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .