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Se deficiency exacerbated AFB 1 ‐induced splenic injury in chicks is related to regulate six selenoproteins and redox/inflammation/apoptotic signaling
Author(s) -
Zhao Ling,
Feng Yue,
Deng Jiang,
Sun Lvhui
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.871.4
Subject(s) - inflammation , apoptosis , redox , microbiology and biotechnology , chemistry , cancer research , immunology , biology , biochemistry , organic chemistry
This study was to explore the mechanism of Se‐mediated protection against AFB 1 ‐induced splenic injury in broilers. Day‐old male chicks (n = 30/group) were fed a Se‐deficient, corn and soy–based diet (BD, 36 μg Se/kg) or the BD + 1.0 mg AFB 1 /kg, 0.3 mg Se/kg, or 1.0 mg AFB 1 /kg with 0.3 mg Se/kg for 3 wk. The Se was added as 2‐hydroxy‐4‐methylselenobutanoic acid. Dietary AFB 1 induced the poor growth performance and spleen injury, decreased ( P < 0.05) body weight, feed intake, feed conversion, and serum interleukin‐1β, increased ( P < 0.05) spleen index and serum interleukin‐6, and decreased the splenic lymphocyte number and white pulp region and histiocyte proliferation in Se adequate groups. Notably, Se deficiency exacerbated ( P < 0.05) these AFB 1 ‐induced alterations. Moreover, Se deficiency reduced ( P < 0.05) splenic glutathione peroxidases (GPX) and glutathione‐S transferases activities and glutathione content in AFB 1 administrated groups. Furthermore, Se deficiency also up‐regulated ( P < 0.05) the antimicrobial ( beta defensin 1 and 2 ) and apoptotic ( Caspase3 ) genes but down‐regulated ( P < 0.05) inflammatory ( E3 ubiquitin‐protein ligase CBL‐B ) genes in AFB 1 supplementation groups. Additionally, Se deficiency down‐regulated ( P < 0.05) Gpx3 , thioredoxin reductase 1 , GPX4 and selenoprotein (SELENO) S and up‐regulated ( P < 0.05) Selenot and Selenou in spleen in AFB 1 administrated groups. In conclusion, dietary Se deficiency aggravated AFB 1 ‐induced splenic injury in chicks, potentially via the regulation of the 6 selenoproteins and oxidative stress, inflammatory and apoptotic signaling. Support or Funding Information Supported in part by Chinese Natural Science Foundation Projects 31772636 and 31501987 and the Fundamental Research Funds for the Central Universities Project 2662018PY030. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .