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A Comparison of Ischemia‐Reperfusion Injuries Induced by Tourniquet and Femoral Artery Ligation in Mouse Hindlimb
Author(s) -
Qian Junliang,
Tu Huiyin,
Zhang Dongze,
Wadman Michael C.,
Li YuLong
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.868.6
Subject(s) - tourniquet , femoral artery , hindlimb , ligation , medicine , ischemia , gastrocnemius muscle , anesthesia , artery , skeletal muscle , surgery , anatomy
The tourniquet and artery ligation are used widely to stop extremity hemorrhage or create a bloodless operating field. However, both of them also induce ischemia‐reperfusion (IR) injuries. To further evaluate the application of the tourniquet and artery ligation, we compared tourniquet and femoral artery ligation‐induced IR injuries in mouse hindlimb. C57/BL6 mice were anesthetized and 3 hours of unilateral hindlimb ischemia was induced by placing an orthodontic rubber band (ORB) at the hip joint or undergoing a surgery procedure of femoral artery ligation, then the ORB tourniquet or artery ligation was released for 24 hours of reperfusion. Immunohistochemistry data demonstrated that tourniquet‐induced IR caused severe skeletal muscle damage, compared to femoral artery ligation‐induced IR. Triphenyl Tetrazolium Chloride Assay further confirmed that the infarct size in the gastrocnemius muscle was higher in tourniquet‐induced IR mice than that in femoral artery ligation‐induced IR. Sciatic nerve‐stimulated gastrocnemius muscle contraction was weak in tourniquet‐induced IR mice (0 g), compared to femoral artery ligation‐induced IR (46.42±3.74 g). Tourniquet‐induced IR also resulted in more serious damage of lymphatic vessels in mouse hindlimb, compared to femoral artery ligation‐induced IR. Our present study indicate that tourniquet‐induced IR causes more severe skeletal muscle injuries than that induced by femoral artery ligation‐related IR. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .