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The Effects of Non‐Small Cell Lung Carcinoma Conditioned Media on Skeletal Muscle Cell Proliferation and Metabolism
Author(s) -
Stott Nicole L,
Beach Cassandra,
Marino Joseph S
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.868.21
Subject(s) - c2c12 , skeletal muscle , cell growth , myocyte , lung cancer , cancer cell , medicine , endocrinology , cancer , cancer research , biology , myogenesis , biochemistry
Lung cancer is the second most common cancer and one of the largest causes of cancer‐related mortality worldwide. 80–85% of all new lung cancer diagnoses are Non‐Small Cell Lung Carcinoma (NSCLC), suggesting a great need to continue elucidating the metabolic mechanisms surrounding this prevalent disease. Due to the oncogenic environment surrounding NSCLC growth, factors released into the environment could have a direct impact on other tissues. In particular, skeletal muscle, a leading insulin‐sensitive and metabolically active tissue, is subject to wasting in many cancer patients. The purpose of this study was to determine how NSCLC conditioned media affects C2C12 skeletal muscle cells proliferation and metabolism. C2C12 mouse skeletal muscle cells were grown for 24 hours and serum‐starved overnight with serum‐free media to induce quiescence. Cells were treated with equal volumes of either fresh growth media or conditioned media from proliferating NSCLC cells. C2C12 myoblast proliferation was assessed every 24 hours for 3 days via an MTT assay using 3‐(4,5‐Dimethyltiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide. Proliferating C2C12 myoblasts treated with fresh growth media or conditioned media from NSCLC cells showed significant proliferation (p=0.0016) over 72 hours. Treatment with NSCLC conditioned media did not induce any significant increases in myoblast proliferation when compared to control C2C12 cells. In conclusion, these data suggest that C2C12 myoblasts did not respond adversely to NSCLC conditioned media. Experiments are ongoing to investigate alterations in metabolic and myogenic signaling pathways, as well as extended time courses. Support or Funding Information This work was supported by the Targeted Research Internal Seed Program, JSM. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .