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Obesity and Chagas Disease: Cardiovascular, Oxidative and Metabolic Aspects of this Relationship
Author(s) -
MartinsPinge Marli Cardoso,
Lucchetti Bruno Fernando Cruz,
Lopes Fernanda Novi Cortegoso,
PingeFilho Phileno
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.836.3
Subject(s) - parasitemia , insulin resistance , medicine , oxidative stress , obesity , blood pressure , p22phox , immunology , endocrinology , physiology , malaria , plasmodium falciparum , nadph oxidase
Chagas disease (CD) is one of the leading neglected tropical infectious diseases, and people living in endemic regions are at potential risk for developing CD. The incidence of obesity has been increasing lately in countries endemic to DC, leading to high blood pressure, insulin resistance, increased levels of inflammation and oxidative stress. This picture promoted by obesity may have effects on the development of CD. In the present study, we evaluated the influence of obesity on acute infection by Trypanosoma cruzi, on cardiovascular, inflammatory parameters and insulin resistance. Obese swiss mice, on the 70th day of life were submitted to intraperitoneal infection with 5×10 2 trypomastigote forms of the Y strain. The cardiovascular parameters were evaluated before and during infection. The parasitemia and the survival up to 30º dpi were analyzed. In the 13th dpi the tissue was collected. Cytokines were determined using commercial kit CBA. Nitric oxide (NO) was determined by the cadmium and Griess technique. The antioxidant capacity and levels of oxidative stress were determined by the ABTS, FRAP, NBT and TBARS assays. Obese animals had higher blood pressure. We observed a higher parasitemia in the infected obese group (OI) compared to the infected control (CI) on days 13 and 15 post infection. All animals in the OI group died until the 19th day after infection while 87.5% of the IC survived to 30º. We observed a drop in mean arterial pressure in OI from the 9th dpi remaining until the end of the evaluation. No changes in blood pressure were found in the IC group. An increase in plasma NO in adipose tissue and aorta in OI was observed. Higher concentrations of INF‐Y and MCP‐1, and a lower concentration of IL‐10 was observed in OI vs CI. It was found a lower insulin sensitivity in obese animals and accentuated after infection. Higher parasitic load was found in adipose and hepatic tissue, and increased levels of oxidative stress in cardiac, hepatic and adipose tissue in the OI group. Our results suggest that the association of obesity and CD during the acute phase promote greater damage to infected animals. Support or Funding Information CAPES (felowship) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .