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Modulation of N ω ‐nitro‐L‐arginine methyl ester (L‐NAME)‐Induced Hypertension and Cardio‐renal Oxidative Stress by Methanol Extract of Persea americana Root
Author(s) -
Adejumobi Olumuyiwa Abiola,
Oyagbemi Ademola Adetokunbo,
Akoleowo Olabimpe Olufunmilayo,
Omotosho Oladipo O.,
Yakubu Momoh,
Omobowale Temidayo Olutayo,
Adedapo Adeolu A.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.835.2
Subject(s) - medicine , oxidative stress , blood pressure , diastole , kidney , endocrinology , cardiology
Hypertension is the leading cause of cardiovascular morbidity and mortality. Uncontrolled hypertension causes end organ damage resulting in hypertensive cardiomyopathy and nephropathy. P. americana root extract (PARE) is commonly used the management of hypertension in Nigeria traditional medicine. In this study, we investigated modulatory effects of PARE on L‐NAME induced hypertension, oxidative stress and cardio‐renal complications Sixty male rats (wistar strain) were used in this study, and they were randomly divided into six experimental groups with ten rats in each group. Group A served as the controls and they received distilled water only for the period of 21 days. Group B was given L‐NAME only at 40mg/kg while groups C, D and E were given a daily dose of L‐NAME at 40 mg/kg and methanol root extract at respective dose of 100mg/kg, 200mg/kg and 400mg/kg for 21 days. Group F was given a daily dose of L‐name+ Lisinopril (0.25mg/kg) orally for 21 days. L‐name was given at 40mg/kg daily. Blood pressure measurements were recorded in conscious animals by tail cuff plethysmography . Biochemical assays were carried out on the serum, cardiac and renal tissues to assess the enzymatic and non‐enzymatic antioxidant defense system. Immunohistochemistry was also done on cardiac and renal tissues. Treatment with L‐NAME caused a significant (p <0.05) increase in systolic, diastolic and mean arterial blood pressure and markers of oxidative stress in cardiac and renal tissues. Immunohistochemistry showed increased expressions of CTn1, NRF2 and NFkB in the heart tissues and KIM‐1, NRF2 and NFkB in the kidney tissues of the group treated with L‐NAME alone compared to the control. Treatment with PARE caused a significant (P<0.05) decrease in blood pressure, an improvement in antioxidant defense systems, reduction in markers of oxidative stress, cardiac and renal injury and inflammation comparable with that of lisinopril.. The results obtained from this study suggest that PARE can modulate high blood pressure with associated end organ damage and cardio‐renal oxidative stress in L‐NAME induced hypertension This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .