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Defining the role of CEBP/α in Functional β‐cell mass
Author(s) -
Smith Courtn J,
Leifer Aaron H,
Ray Jason D.,
Tessem Jeffery Sivert
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.834.9
Subject(s) - gene knockdown , endocrinology , medicine , adipogenesis , transcription factor , cell growth , diabetes mellitus , biology , microarray analysis techniques , cancer research , adipose tissue , gene expression , gene , biochemistry
Metabolic diseases, such as obesity and diabetes, continue to rise exponentially across the globe. More than 68.8% of adults are considered to be overweight or obese, with more than 14% of Americans suffering from diabetes. Prolonged states of hyperlipidemia and hyperglycemia are characteristic phenotypes of diabetes. Identifying genes involved in the regulation of blood glucose and adipogenesis are essential for establishing a more comprehensive treatment for diabetes. CCAAT‐enhancer binding protein‐α (CEBPα) has been shown to play a key role in hepatic regulation of blood glucose and adipogenesis. Inactivating CEBPα, results in dysregulation of adipose tissue browning and inhibition of glycemia regulation. These data demonstrate that CEBPα expression is necessary for energy metabolism. We have previously shown that the transcription factor Nkx6.1 is sufficient to upregulates genes necessary for inducing β‐cell proliferation, increasing cell survival and improving insulin secretion. Our previous microarray indicated a potential interaction between Nkx6.1 and CEBPα. We hypothesize that CEBPα is an intermediate which is necessary for Nkx6.1 to increase functional β‐cell mass. Here we demonstrate the effect of CEBPα mediated β‐cell proliferation, insulin secretion and cell survival. Furthermore, we demonstrate that knockdown of CEBPα downregulates c‐Fos, which is required for Nkx6.1 enhancement of functional β‐cell mass. These data demonstrate the role of CEBPα in increasing functional β‐cell mass. Support or Funding Information ADA 1‐17‐IBS‐101 to JST This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .