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Effect of fructooligosaccharides on apoptosis and inflammation in enteroendocrine cells
Author(s) -
Tuangkijkul Nuttha,
Wongkrasant Preedajit,
Muanprasat Chatchai
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.834.4
Subject(s) - apoptosis , inflammation , ampk , cancer research , enteroendocrine cell , microbiology and biotechnology , tumor necrosis factor alpha , biology , medicine , immunology , endocrinology , protein kinase a , kinase , biochemistry , hormone , endocrine system
Type 2 diabetes mellitus (T2DM) is considered as a chronic inflammatory disease. It has been found that TNF‐α‐induced inflammation of intestinal L‐cells leading to apoptosis that may cause a decrease in GLP‐1 secretion. Therefore, inhibition of inflammation‐mediated L cell apoptosis may provide the beneficial effect to T2DM patients. Most of prebiotics is known to suppress intestinal inflammation. This study aimed to investigate whether fructooligosaccharides (FOS), one of probiotics, on TNF‐α‐induced L cell inflammation and apoptosis. Indeed, the mouse secretin tumor cell line (STC‐1 cell) was used as an in vitro model of enteroendocrine L cells. We hypothesized that FOS might inhibit inflammatory signals via AMP‐activated protein kinase (AMPK), an intracellular energy sensor that known to suppress intestinal inflammation, which might result in inhibition of apoptosis. Immunofluorescence staining revealed that FOS did not suppress TNF‐α‐induced NF‐κB nuclear translocation in STC‐1 cell. Using cell death assay, Hoechst 33342 and ethidium homodimer‐1 co‐staining was performed. It was found that FOS significantly reduced number of apoptotic STC‐1 cells via AMPK‐independent mechanism. In conclusion, FOS may be useful for recovering the function of GLP‐1‐secreting cells that may be beneficial for T2DM patients. Support or Funding Information This work is supported by Tonkla Ramathibodi program, Faculty of Science Mahidol University and Thailand Research Fund and Mahidol University (BRG5980008) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .