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Effects of Nicorandil and Chronic Physical Exercise on Glycemia in Diabetic Rats Induced by Streptozotocin
Author(s) -
SánchezDuarte Elizabeth,
SánchezDuarte Sarai,
VeraDelgado Karla S.,
MontoyaPérez Rocío,
CaudilloCisneros Cipriana,
Cordova Victor,
SánchezBriones Luis A.,
MárquezGamiño Sergio
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.834.12
Subject(s) - medicine , nicorandil , endocrinology , diabetes mellitus , streptozotocin , insulin , insulin resistance
Diabetes is a chronic metabolic disease characterized by persistent hyperglycemia caused by a decrease or deficiency of insulin secretion and/or peripheral insulin resistance. Regular physical exercise could improves glucose metabolism and insulin sensitivity, which is essential for preventing diabetic complications. Moreover, there is evidence that the opening of ATP sensitive potassium (K ATP ) channels contributes to cytoprotective effects under metabolic stress, therefore K ATP channels could be a therapeutic target in this disease. Thus, the purpose of this study was to assess the impact of the potential effects of nicorandil (K ATP channel opener) after a chronic exercise training program on levels glicemic in diabetic rats. Male Wistar rats (23–25 days old) were randomly divided into six groups, among them: sedentary control, trained control, sedentary diabetic, trained diabetic, sedentary diabetic plus nicorandil, trained diabetic plus nicorandil. Diabetes was induced by a single streptozotocin injection (100 mg/kg body weight), animals with fasting blood glucose levels ≥ 250 mg/dL were considered as diabetic. Training comprised 8 weeks of treadmill running (30 minutes daily, 5 days/week); at week 4, nicorandil (3 mg/kg) was administered for to 4 weeks. Fasting blood glucose (FBG) level was recorded every week for 8 consecutive weeks. At the end of experimental protocol, insulin tolerance test (ITT) was evaluated in all groups, rats received a application of insulin (0.75UI/kg body weight), with blood samples drawn after 0, 30, 60, 90 and 120 min postinjection. Blood glucose response to ITT was calculated as area under the curve (AUC‐ITT) of the blood glucose excursion along 120 minutes after intra‐peritoneal insulin injection and correlated with the corresponding fasting values according to the trapezoidal method. Treatment with nicorandil and the chronic exercise exerted remarkable effects on hyperglycemic status in diabetic rats by decreases FBG levels (47.51± 10.2 %) and inducing significant increases in the insulin sensitivity (P< 0.05) compared with sedentary diabetic rats and trained diabetic rats. Our results suggest than opening KATP channels by nicorandil mediates potential benefits the chronic physical exercise on glycemic in diabetes. Support or Funding Information We thank Universidad de Guanajuato, Campus León. We acknowledge the financing PRODEP (Programa para el Desarrollo Profesional Docente en Educación Superior, ESD (511‐6/18‐8876). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .