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The Protective Effects of Phosphodiesterase 5 Inhibitors "Sildenafil & Ordonafil” on Ischemia‐Reperfusion Injury
Author(s) -
Khatib Said Younes
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.833.7
Subject(s) - sildenafil , cgmp specific phosphodiesterase type 5 , ischemia , creatine kinase , pharmacology , phosphodiesterase , reperfusion injury , vardenafil , cardioprotection , chemistry , perfusion , ventricle , medicine , cardiology , enzyme , tadalafil , biochemistry
Phosphodiesterase type‐5 (PDE‐5) inhibitors, Sildenafil (SILD), Vardenafil (VAR) and Ordonafil (ORD), have been developed for erectile dysfunction. Recently SILD and VAR were shown to have cardioprotective effect by reducing the infarct size in ischemic/reperfusion injury. To support the suggestion that this effect is a generalized "class" effect we decided to test the cardioprotective effect of ORD and to compare it to that of SILD. Isolated rabbit's hearts, perfused using Langendorff system, were subjected to 30 min of global ischemia followed by 2 hrs of reperfusion. At end of perfusion the heart was sliced into 5–6 cuts, 2 mm thick and incubated in 1 % triphenyltetrazolium chloride. The area of infarction and the whole left ventricle (LV) were measured by computer morphometry using image tool software. The infarct size was expressed as % of LV. Creatine kinase enzyme (CK) as a marker of infarction, was determined in the perfusate pre‐ and post‐ischemia by enzyme bioassay. The drugs, SILD or ORD at 1 mg/L, perfused the heart for 5 min before induction of ischemia. The results of both drugs showed significant cardioprotective effect and the infarct size ( as % of LV) was reduced from 38 ± 12 % in the control group to 5±2 and 9±6 %, mean±SD, n=6–8, P<0.004 for ORD and SILD respectively). Similarly the increase in CK was attenuated from 32 times increase in the control group to 20 times and 26 times for ORD and SILD respectively. Theses results support the notation that the class of PDE‐5 inhibitors has protective effect in the ischemic heart beside their effect in the treatment of erectile dysfunction. Support or Funding Information Deanship of research This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .