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The role of Kv3 channels in regulating epithelial mesenchymal transition
Author(s) -
Sim Hunju,
Lee So Yeong
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.824.8
Subject(s) - epithelial–mesenchymal transition , mesenchymal stem cell , vimentin , cancer research , cytokeratin , cancer , biology , medicine , microbiology and biotechnology , metastasis , immunohistochemistry
Epithelial mesenchymal transition (EMT) and mesenchymal epithelial transition (MET) are closely related to cancer progression. EMT is demonstrated to alter cell adhesion and cell motility. Recently, it has been suggested that ion channels, including voltage‐gated potassium (Kv) channels are also involved in a consequence of EMT. Dogs are recognized as effective models of cancer development and in clinical therapeutic trials. In the present study, we used canine breast cancer cells, CHMp, which have mesenchymal characteristics, to investigate the involvement of Kv3 channels in the EMT/MET processes. We found that treatment with blood depressing substance II (BDS), which is a specific Kv3 subfamily blocker, increased the expression of cytokeratin‐18, an epithelial cell marker and decreased the expression of vimentin, a mesenchymal cell marker. These results suggest that blockade of Kv3 using BDS induced MET in CHMp cells. Future research is needed to elucidate the specific mechanisms governing the relationship between Kv3 and EMT/MET. Support or Funding Information This research was supported by the Bio and Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science, ICT and Future Planning (NRF‐2016M3A9B6026771). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .