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Hydrolyzed Rutin in Colon Cancer Chemoprevention: Is an Epigenetic Effect of Flavonoid?
Author(s) -
Priolli Denise Gonlaves,
Martinez Natalia Peres,
Castilho da Silva Daniel,
Scobar Leticia,
Mancilha Bárbara Vilela,
Castro Camila Maria,
Klinkerfuss Natália Táis,
Ribeiro Marcelo Lima,
Longato Giovanna Barbarini,
Ortega Manoela,
Oliveira Carvalho Patricia
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.816.14
Subject(s) - epigenetics , rutin , cancer research , biology , biochemistry , gene , antioxidant
Colorectal cancer, one of the most common malignant tumors, is generally a disease caused by genetic and epigenetic disruptions mutation of colonic cells. Novel therapies based on interfering in epigenetic pathways could represent a significant advance in tumors prevention. Epigenetics describes alterations of gene expression and chromatin organization without changes in DNA sequence. Dietary compounds possess the propriety of changing epigenetic processes and gaining attention in cancer studies. The flavonoids are part of the list of the epigenetic drugs in clinical trials. However, studies with modified flavonoids to changing epigenetics processes are lacking. Ataxia telangiectasia (ATM) and Rad3‐related (ATR) proteins are key regulators of the DNA damage response (DDR) and maintain genome integrity in eukaryotic cells, then the correlation with epigenetics activity is plausible. In the present study, we showed the effect of hydrolyzed rutin, a modified flavonoid compound, in colon carcinoma chemoprevention. Our results demonstrated that hydrolyzed rutin inhibits the colon carcinoma growth by inducing G0/G1 arrest in the cell cycle. Additionally, hydrolyzed rutin leads the phosphorylation of NF‐Kβ, a process involved in epigenetic changes. The NF‐Kβ phosphorylation was accompanied by the p53‐protein functional return in chemoprophylactic. The results suggest the colon carcinoma growth could be influenced by hydrolyzed rutin ATM signaling pathway. The figure shows the key pathway influenced by Hydrolyzed Rutin in colon cancer. Hydrolyzed Rutin leads the phosphorylation of NF‐kβ accompanied by the p53‐protein functional return, a process involved in epigenetic changes. To our knowledge, this study is the first to evaluate the antiproliferative effect of hydrolyzed rutin in vivo as a chemoprophylactic approach for colon carcinoma. Support or Funding Information São Paulo Research Foundation (FAPESP) to DGP (grant numbers # 2015/07891‐2 and 2012/04634‐1).Key pathway influenced by Hydrolyzed Rutin in colon cancer. Hydrolyzed Hydrolyzed Rutin leads the phosphorylation of NF‐kβ accompanied by the p53‐protein functional return, a process involved in epigenetic changes.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .