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Serum Estradiol/Testosterone Ratio Affects the Development of Benign Prostate Hyperplasia in Rats
Author(s) -
Liu Xiangyun,
Li Kai,
Liu Zhiwei,
Gui Shukang
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.802.72
Subject(s) - hyperplasia , prostate , testosterone (patch) , medicine , h&e stain , endocrinology , urology , prostate cancer , staining , immunohistochemistry , pathology , cancer
Objective Benign prostatic hyperplasia (BPH) develops more likely with increasing age and changing serum concentrations of circulating estradiol (E2) and/or testosterone (T). Methods In this study we explored the relationship between serum E2/T ratio and BPH risk in rats by fitting a mathematical model. A total of 176 male rats were randomized divided into normal control, castrated control, and 20 more groups of castrated animals were administered subcutaneously once daily with one of the four dose levels (0.06, 0.17, 0.5, and 1.5 mg per rat) of T combined with one of the five dose levels (2, 6, 17, 50, and 150μg/kg) of E2 in olive oil by different dose ratio. After 30 days, Serial blood samples were obtained to determine serum T and E 2 levels by magnetic bead enzyme‐linked immunosorbent assay. The volume of prostate was measured by water displacement method. The prostate specimens were then fixed, embedded in paraffin, and subjected to hematoxylin and eosin staining for pathological examination. MATLAB software was used to simulate the relationship between prostate/body weight ratio (PBR) and E 2 /T ratio with a mathematical equation. Results Compared with the normal control group, the prostate volume decreased significantly (P < 0.05) in the castrated control group and the treatment groups receiving E2/T dose of 2/0.06 or 6/0.06, while the prostate volume increased significantly (P < 0.05) in the other treatment groups. Compared with the normal control group, the PBR increased statistically significant in the treatment groups receiving E2/T dose of 50/0.5, 150/0.5, 2/1.5, 6/1.5,17/1.5, 50/1.5, or 150/1.5 (P < 0.05). The values of PBR, E 2 and T in the treatment groups were significantly higher than those in the control groups. Stepwise regression showed that PBR was a function of E 2 and T. PBR=−0.1782 + 0.0081E 2 + 0.063T − 0.6 × 10 −5 E 2 2 − 0.28 × 10 −3 T 2 , (R 2 =0.6317) Conclusion E 2 /T ratio change may be one of the risk factors for PBR, which is associated with the development of BPH. Support or Funding Information National Natural Science Foundation of China (No.: 81772429) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .