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Evaluation of the oncolytic ability of Theiler's murine encephalomyelitis virus in glioblastoma cell lines GL26 and GL261
Author(s) -
Grey Devon,
Freundt Eric C.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.802.55
Subject(s) - oncolytic virus , biology , cell culture , olig2 , virology , glioma , cell , virus , cancer research , oligodendrocyte , genetics , myelin , neuroscience , central nervous system
Glioblastoma multiforme (GBM) is type of brain cancer for which there are no effective treatments. Oncolytic viruses, such as poliovirus, are currently being tested as potential therapeutics in GBM. We recently found that Theiler's murine encephalomyelitis virus (TMEV), a relative of poliovirus, specifically downregulates expression of four transcription factors, known as OLIG2, POU3F3, SOX2, and SALL2 that are required for proliferation of GBM cells. However, these studies were done in stem‐cell derived oligodendrocyte progenitor cells and it is not known if TMEV can disrupt expression of these genes in GBM cells. In this study, we have infected the glioma cell lines GL26 and GL261 with two strains of TMEV, known as DA and GDVII and evaluated infection efficiency by immunofluorescence microscopy. While glioblastoma cells were susceptible to both strains, DA infected with greater efficiency than the GDVII strain. These differences were not observed in a fibroblast cell line, BHK 21 cells, which were equivalently infected by both strains. Chimeric viruses were used to determine whether strain‐specific differences in infectivity were due to capsid proteins of viral nonstructural proteins. Additionally, we assessed the oncolytic capacity and the ability of TMEV to downregulate OLIG2 in GBM cells. These results suggest that TMEV serves as a model to understand how picornaviruses can limit GBM proliferation. Support or Funding Information DG was supported by the Office of Undergraduate Research and Inquiry Student Research Grant This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .