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Microstructure Detection by Scanning Acoustic Microscope (SAM) using Special and Antigen Staining
Author(s) -
Miura Katsutoshi,
Fujie Michio,
Yamashita Kanna
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.802.1
Subject(s) - elastin , staining , immunostaining , extracellular matrix , chemistry , antigen , microbiology and biotechnology , pathology , biology , immunohistochemistry , immunology , biochemistry , medicine
Objective A SAM detects and visualizes cellular components and extracellular matrix (ECM) proteins. However, which materials contribute to the values of speed‐of‐sound (SOS) or attenuation‐of‐sound (AOS) is unknown. SOS and AOS indicate tissue stiffness or softness because greater SOS and AOS areas correspond to stiffer portions of tissues. The aim of this study is to clarify the role of each component in SAM observation. Methods To detect each component, special staining for ECM and cellular components such as collagen, elastin and DNA were used. To recognize specific antigens, immunostaining with Ni‐DAB solution as horseradish peroxidase substrate was applied. SAM images before and after staining were compared. Results For nuclear detection, both hematoxylin and Giemsa staining resulted in increased SOS and AOS values of the nuclei (Fig. 1 and Fig. 2). To distinguish ECM components, aniline blue (Fig. 3) and Sirius red for collagen and resorcin‐fuchsin (Fig. 4) for elastin stained well to increase SOS and AOS values according to duration of incubation. After collagenase digestion, SOS and AOS values at the positive‐staining areas decreased. Immunostaining with Ni‐DAB solution raised SOS and AOS values of antigen location (Fig. 5). Conclusions Special and immunostaining used in light microscopy are also available to certain the antigen location for SAM. Collagens and smooth muscles contribute to raise the values of SOS and AOS. Support or Funding Information The study was supported by a grant from the Japan Society for the Promotion of Science KAKENHI, Scientific Research Grant Number (c) 15K08375. There are no conflicts of interest to declare. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .