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GlycoSense™: A Simple Alternative to Existing Methods for Glycosylation Detection and Monitoring
Author(s) -
Woods Robert J.,
Saunders Matthew J.,
Yang Loretta
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.801.4
Subject(s) - glycan , glycosylation , multiplex , glycoprotein , computational biology , reagent , computer science , chemistry , combinatorial chemistry , biochemistry , nanotechnology , biology , bioinformatics , materials science , organic chemistry
Lectenz ® Bio has developed an innovative technology called GlycoSense™ that detects binding between glycans and glycan‐specific reagents that are conjugated to spectrally‐unique beads. By combining the individual bead‐based reagents into a multiplex array, a profile of key glycan features can be obtained in less than a minute on a basic cytometer. We illustrate the performance of the method using glycoprotein standards and demonstrate its utility by monitoring changes in glycosylation patterns upon treatment with glycoenzymes. The GlycoSense™ approach addresses the unmet need for a rapid and robust tool to analyze protein glycosylation. In addition to established carbohydrate‐detection reagents such as antibodies and lectins, this approach can employ the engineered proteins (known as Lectenz ® ), developed by Lectenz ® Bio. Support or Funding Information NIH U01 CA221213, R43 GM123863 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .