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The Dual Specific Hemolysin‐X is a Bioactive Natural Product that Forms Pores on the Surface of Erythrocytes
Author(s) -
Dam Tarun,
Welch Christina,
Edwards Jared,
Kadav Priyanka,
Hromada Michael,
Bandyopadhyay Purnima
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.798.5
Subject(s) - hemolysin , lysis , glycoprotein , chemistry , ligand (biochemistry) , natural product , biophysics , biochemistry , microbiology and biotechnology , biology , receptor , gene , virulence
Hemolysins are natural cytotoxic agents that are capable of destabilizing plasma membrane structures of erythrocytes. We recently identified a plant hemolysin named H e mo l y sin‐ X (HelyX) that showed dual specificity for cholesterol and certain serum glycoproteins, such as asialofetuin and fibrinogen. HelyX was isolated through two different approaches: protein purification protocols as well as organic solvent extraction. Ligand binding properties of HelyX were elucidated by inhibition assays using microtiter plates as well as spectrophotometer. HelyX demonstrated significantly fast kinetics (30–180 seconds) of lysis and ligand binding. Scanning electron microscopic imaging captured distinct pores on the surface of HelyX‐treated erythrocytes. Our results indicate that HelyX disintegrates mammalian red blood cells by forming pores on their surfaces. Support or Funding Information Michigan Technological University This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .