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The acyltransferase Gpc1 impacts PC molecular species and phenotypic outcomes
Author(s) -
Nikiforov Alexiy,
Skinner Brelan,
Godshalk Maeve,
PattonVogt Jana
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.796.10
Subject(s) - lysophosphatidylcholine , phosphatidylcholine , phospholipid , chemistry , biochemistry , membrane , acyltransferase , lysophospholipase , popc , enzyme , phospholipase
Phospholipids are major components of cellular membranes and their biochemical properties, such as fatty acid composition, help define the biophysical properties of membranes. Phosphatidylcholine (PC) is the most abundant phospholipid in cellular membranes and is synthesized primarily by two well defined metabolic pathways in Saccharomyces cerevisiae . Our laboratory recently characterized a novel glycerophosphocholine (GPC) acyltransferase, encoded by GPC1 , that provides a third route for PC biosynthesis and is involved in the newly defined PC deacylation‐reacylation remodeling pathway (PC‐DRP). PC‐DRP begins with GPC, the product of complete PC deacylation by phospholipases of the B type. Gpc1 acylates GPC to form lysophosphatidylcholine (LysoPC). LysoPC can subsequently be converted to PC by Ale1. Previous results have shown that Gpc1 prefers the addition of saturated fatty acids during PC remodeling, which results in more saturated PC species and has the potential to influence the biophysical properties of the membrane. To better understand how GPC1 is regulated and to determine its physiological role in the cell, we assessed the effect of GPC1 dosage and temperature alterations on growth, expression, and PC molecular species. Preliminary data indicates that while increasing the temperature from 30°C to 37°C slows growth in a wild type strain, the gpc1Δ mutant is much less effected. As expected, overexpression of GPC1 further negatively impacts growth at 37°C. GPC1 message abundance, as measured by qRT‐PCR, is decreased at 37°C as compared to 30°C in a wild type strain. Other members of PC‐DRP, including ALE1 and PLB1 also display decreased expression upon elevated temperature. Mass spectrometry‐based analysis of the PC molecular species under these conditions are ongoing. Other stressors expected to impact membrane events, including inositol availability, are being examined. These experiments will provide insight into the physiological role of Gpc1. Support or Funding Information NIH R15 GM104876 (to JPV) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .