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Ferric Reductase Knockdown in Drosophila S2 Cells
Author(s) -
Arismendiz Omar Alexis Rodriguez,
Holst Jessica,
Ochoa Alma,
Gorman Maureen J.,
Ragan Emily J.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.795.15
Subject(s) - hemolymph , gene knockdown , ferrous , ferric , transporter , ferritin , rna interference , transferrin , dmt1 , transferrin receptor , drosophila melanogaster , microbiology and biotechnology , reductase , biochemistry , biology , chemistry , enzyme , gene , rna , organic chemistry
Iron is an essential micronutrient that is potentially harmful due to its redox properties, which is why its transport between cells must be a highly regulated process. As compared to mammals, very little is known about iron uptake in insects, because receptors for ferritin or transferrin have not been identified in insects. The goal of the proposed research is to understand the physiological mechanisms of iron transport in insects. We are testing the hypothesis that the reduction of iron ions in the hemolymph leads to iron transport into cells. Hemolymph contains iron in the ferric form, but iron transporters in animal cells are specific to ferrous iron; therefore iron uptake via an influx transporter is likely to require a reduction step. We silenced a putative ferric reductase CG8399 by RNA interference (RNAi) to determine if it contributes to ferric reductase activity and impacts the iron content of the cells. We analyzed the efficiency of knockdown of CG8399 by real time, quantitative PCR (qPCR). Support or Funding Information NSF IOS‐1656407 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .