Premium
Facilitating fructose‐driven metabolisms increases a capability to resist anoxic stress in Drosophila
Author(s) -
Kim Man Su,
Kim Hongjo,
Kim Misun
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.794.6
Subject(s) - anoxic waters , fructose , glycolysis , drosophila melanogaster , biology , microbiology and biotechnology , gene , fight or flight response , biochemistry , enzyme , ecology
Hypoxic stress is linked to various cardiovascular disorders including stroke and myocardial infarction. A key pathophysiological process upon hypoxic stress is a reduction in ATP generation. However, a fructose‐driven glycolysis has been proposed as an alternative biochemical pathway, which could sustain ATP production even in anoxic stress. Here we tested the hypothesis that facilitating fructose‐driven ATP generation increases organisms' capability to withstand anoxic stress using a Drosophila as an in vivo animal model. Genetically modified flies that contain human fructose transporter (GluT5) and ketohexokinase (KHK) sequence downstream of UAS were constructed. Using a GAL4‐UAS system that allows us to express GluT5 and KHK in a tissue‐ and time‐dependent manner, it was found that overexpression of both genes in whole flies (with Act5c‐gene switch GAL4 driver) or in neurons (with elav‐gene switch GAL4 driver) reduces flies' mortality upon anoxic stress. Besides, it was found that spontaneous locomotor activity of the GluT5/KHK overexpression flies was higher than that of control flies upon anoxic assaults. Taken together, these data suggest that enhancing fructose metabolisms could be utilized as a potentially clinically relevant approach to minimize hypoxia‐induced cellular damages. Support or Funding Information This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and Technology (NRF‐2017R1E1A2A01079316) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .