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Measuring T‐cell avidity and enrichment using an acoustic force‐based technology
Author(s) -
Scheper Wouter,
MerinoRodriguez Elena,
Braster Rens,
Sitters Gerrit,
Oswald Felix,
Schumacher Ton,
Candelli Andrea
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.792.12
Subject(s) - avidity , immune system , melanoma , population , cell , antigen , immunology , t cell , microbiology and biotechnology , antibody , chemistry , biology , cancer research , medicine , biochemistry , environmental health
The key driver for effective immunotherapies is the overall strength binding of the immune cell and the target cell(e.g. tumor cells). The overall strength is known as ‘avidity’, a parameter reflecting interaction efficiency. One of the main obstacles has been the generation of immunotherapies promoting effective and long lasting immune responses, due to the lack of tools measuring this parameter. Here, we have made used of acoustic forces as a novel method to isolate immune cells based on their avidity to specific targets, such as (tumor) cells. The force that cell target need to experience to be separated is called ‘rupture force’. In this study, we were able to identify the rupture force of tumor specific and non specific T cells and separate these different populations applying controllable acoustic forces. T cells engineered with a melanoma antigen recognizing T cell receptor needed 6 times more relative force than the control, nonspecific T cells, to be separate from their melanoma cells. These findings indicate that the specific melanoma T cells bind with higher efficiency and higher avidity than the nonspecific ones, and that they can be separated with this method. Furthermore, 1.4 to 3.6‐fold enrichment of high avidity T cells from a mixed specific:nonspecific (1:10) T cell population was obtained after their isolation using acoustic forces and confirmed using FACS measurements. Therefore, we demonstrate here that acoustic forces can be used as a tool to measure avidity. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .