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Exosomes Promote a Metastatic Mircroenvironment in Triple Negative Breast Cancer
Author(s) -
Roberts Paige A,
Coward Jordon A,
Johnson Jasmine P,
Reeves Alexavier,
Lemieux KiTani P
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.789.5
Subject(s) - microvesicles , triple negative breast cancer , cancer research , matrigel , metastasis , cell growth , exosome , cancer cell , tumor microenvironment , cell migration , cell , chemistry , biology , breast cancer , cancer , medicine , angiogenesis , microrna , biochemistry , tumor cells , gene
Triple negative breast cancer (TNBC) is the most aggressive subtype and is characterized by its patterns of metastasis and abnormal morphology. Due to the molecular heterogeneity in TNBC's, discovering effective targeted therapies has proven to be difficult. Our research group has designed a series of experiments to gain insight on how role of the microenvironment plays a role in TNBC proliferation, migration and invasion. Studies indicate that cellular stress causes exosomes to be released from both tumorigenic and nontumorigenic cells. Given that exosomes are systemic cell‐to‐cell transporters, our research goal is to better understand the role of exosomes harvested from non‐tumorigenic breast epithelial cells have on TNBC cells by measuring the proliferation, migration, and invasion when TNBC cells are exposed to these exosomes. Exosomes were harvested and purified from MCF‐10A cells. Proliferation was measured by plating 5 × 10 5 MDA‐MB‐231 cells in a 96 well plate which were exposed to either exosomes from MCF‐10A cells or growth media. Absorbance was measured at 560nm after 48 hours. Migration was measured by plating 5 × 10 MDA‐MD‐231 cells with the exosomal media or growth media for 48 hours. Invasion was measured using the Matrigel assay at a density of 5 × 10 5 MDA‐MB‐23 cells were exposed to exosomes from MCF10A cells. The migration and invasion assays were measured by counting cells at 4x magnification. Our data indicate that MCF‐10A exosomes increased the growth of TNBC MDA‐MB231 cells by an average of approximately 30% compared to the control (p>0.05). The MDA‐MB‐231 cells demonstrated enhanced migration and invasion when exposed to exosomes from MCF‐10A cells at 10% and 4%, respectively (p> 0.05) when compared to the control. Exosomes from nontumorigenic breast epithelial cell line MCF‐10A cells caused increases in proliferation, migration, and invasion in TNBC cell line MDA‐MB‐231. Our data suggests that exosomes are key mediators in the progression of cancer. Support or Funding Information D34HP00006‐31‐01 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .