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Tracking and Detection of Bactericidal Quantum Dots
Author(s) -
Untracht Zachary,
Ozcan Ali,
Santra Swadeshmukul,
Kang Hyeran
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.785.12
Subject(s) - nanoparticle , sodium dodecyl sulfate , chemistry , nanotechnology , biocide , chromatography , materials science , organic chemistry
Zinkicide is a bactericidal nanoparticle that combats Citrus Greening with exceptional field efficacy. Bactericidal nanoparticle detection is important for surpassing EPA criteria and bringing biocide products to market; however quantitative analysis on Zinkicide degradation in particular is currently lacking. Here, we have developed a protocol using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) to monitor and track bactericidal nanoparticles in varying solutions, including water and acidic buffer mimicking citrus pH. The nanoparticle detection was performed using UV fluorescent imaging of polyacrylamide gels. This assay is a cost‐effective and relatively simple approach for detecting and quantifying the intensity, concentration, and molecular weight change of nanoparticles. We observed intensity fluctuations of Zinkicide over time, indicating that the capping agent of the nanoparticle may be affected. Our results show that citric acid buffer induces the greatest degradation of Zinkicide evidenced by molecular weight changes. These findings are beneficial for general tracking and detection of bactericidal quantum dots. Support or Funding Information The authors would like thank the Kang Lab members for their continued support and assistance. This study was supported by the UCF start‐up fund (Kang). Authors acknowledge the UCF Materials Innovation and Sustainable Agriculture Center for facilities and technical support. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .