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Regulation of RpoS by RbsD in Escherichia coli
Author(s) -
Peterson Celeste,
Dorlean Shaina,
Parsons Dylan,
Tran Thomas,
Dey Arup
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.778.13
Subject(s) - rpos , lac operon , biology , escherichia coli , microbiology and biotechnology , gene , genetics , gene expression , promoter
In stressful environments, RpoS a stationary phase regulator, accumulates in E. coli and induces a state of dormancy. A screen for regulators of RpoS was carried out with an overexpression DNA library and an RpoS’‐’LacZ fusion. Overexpression of the rbsD gene, which codes for a ribose pyranase necessary for efficient metabolism of ribose, was found to reduce RpoS levels. We next used a series of LacZ fusions and RT‐qPCR to determine how rbsD affects RpoS. We found that rpoS mRNA levels were modestly lowered in the rbsD overexpressed strain. However, a much larger effect was seen in the post‐transcriptional level as determined by an RpoS’‐’LacZ protein fusion. Furthermore the effect of overexpression of rbsD was diminished in a fusion that did not have the RpoS untranslated hairpin loop. In summary overexpression of rbsD resulted in decreased RpoS translation in a manner that depended on the RpoS hairpin loop. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .