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Circularization of linear chromosomes and telomerase RNA gain‐of‐function mutations in Saccharomyces cerevisiae
Author(s) -
Mefford Melissa Anne
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.776.5
Subject(s) - telomerase , telomere , saccharomyces cerevisiae , ribonucleoprotein , biology , genetics , eukaryotic chromosome fine structure , telomerase rna component , rna , dna , dna replication , yeast , telomerase reverse transcriptase , computational biology , gene
Telomeres are repeated DNA sequences located at the ends of linear eukaryotic chromosomes. While they act as protective caps, they cannot be fully copied by the DNA replication machinery. To overcome this end‐replication problem, most eukaryotic organisms express the ribonucleoprotein enzyme complex telomerase. I will present preliminary results investigating basic, broad questions of telomere and telomerase evolution in the budding yeast, Saccharomyces cerevisiae . First, we are genetically engineering strains with circularized versions of each of the 16 chromosomes to assess the viability and potential fitness effects on the organism. Second, we are screening for gain‐of‐function mutations in telomerase RNA to gain further insights into how this component contributes to enzyme function. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .