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Tissue quantification of neutral and acid mucins in the mucosa of the colon submitted to 10 days of gastric restriction in an animal model
Author(s) -
Akamatsu Flávia Emi,
Fontes Luiz Gustavo,
Itezerote Ana Maria,
Saleh Samir,
Teodoro Walcy Rosolia,
Hojaij Flávio,
Andrade Mauro,
Pereira José Aires,
Martinez Carlos Augusto Real,
Jacomo Alfredo Luiz
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.767.32
Subject(s) - mucin , stomach , goblet cell , medicine , gastroenterology , pathology , biology , epithelium
Background Obesity and its related comorbidities have detrimental effects for the affected individual and pose a major challenge on public health systems worldwide. However, mechanisms of surgical weight loss and long‐term consequences of bariatric surgery remain unclear. Gastric reduction procedures are currently employed to treat morbid obesity and there are few reports concerning the consequent effects in large intestine. Purpose To quantify the expression of neutral and acids mucins in mucosa of the colon in animal model and correlate this to gastric restriction Methods Experimental model of gastric restriction was devised using rats. The animals were submitted to surgical gastrostomy and a cylindrical loofah was inserted into the stomach. We studied 30 adult male Wistar rats divided in three groups: the stomach reduction group (R10), the sham group (S10), which underwent the same procedure except for the loofah insertion and the control group (C10). Animals were fed and kept in separate cages. They were weighed every other day until being sacrificed on day 10. The expression of neutral and acid mucins was evaluated using the histochemical techniques of Periodic Acid Schiff and Alcian Blue, respectively and Picrosirius Red. The tissue mucins and collagen expression were quantified by computer‐assisted image analysis software. Goblet cells and protein content were presented by groups as mean, median, minimum and maximum and it was compared between groups using generalized estimation equations (GEE) with normal distribution and identity link function supposing exchangeable correlation matrix between fields. Bonferroni's multiple comparison was applied to identify differences between groups. The results are described by the mean value with respective standard deviation. We used Spearman correlation to verify correlation between weight, height of the crypt and cells quantity. All tests were applied with 5% significance level. Results There was increased expression of neutral mucins and acid mucins as well as Goblet cells in the R10 group. Picrosirius Red enhanced total collagen in restriction group. Conclusions Gastric restriction for 10 days in animal model increased neutral mucins and acid mucins as well as collagen content with trophic maintenance. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .