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Angiopoietin‐1 Protects 3T3‐L1 Pre‐Adipocytes from Saturated Fatty Acid‐Induced Cell Death
Author(s) -
Son Yura,
Paton Chad Michael
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.752.3
Subject(s) - adipocyte , adipogenesis , angiogenesis , microbiology and biotechnology , angiopoietin , receptor , apoptosis , chemistry , medicine , crosstalk , endocrinology , biology , adipose tissue , biochemistry , vascular endothelial growth factor , vegf receptors , physics , optics
Crosstalk between endothelial cells and adipocytes is vital to adipocyte functions, but little is known about the mechanisms or factors controlling the process. Angiogenesis is a critical component linking the endothelium to healthy adipogenesis, yet it is not known if or how it is involved in adipocyte physiology. Therefore, the purpose of this study was to determine the effect of Angiopoietin‐1 (Ang‐1) and ‐2 (Ang‐2) as well as their receptor, Tie‐2, on adipocyte physiology. 3T3‐L1 pre‐ and mature adipocytes were found to express Ang‐1, Ang‐2, and Tie‐2 which decrease upon PUFA‐treatment. Furthermore, 3T3‐L1 cells treated with recombinant Ang‐1 or Ang‐2 increased expression of the anti‐apoptotic gene Bcl‐x and decreased expression of the pro‐apoptotic gene Casp‐8. Next, pre‐adipocytes were treated with saturated fatty acids (SFA) to induce cell stress. SFA‐mediated splicing of X‐box‐binding protein‐1 (Xbp‐1s) was reduced by co‐treatment with Ang‐1 and cell viability was improved in the presence of SFA + Ang‐1. Taken together, these results indicate that Ang‐1 may protect pre‐adipocytes from SFA‐induced apoptosis and ER stress. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .