Premium
MARCKS Expression and Localization in the Aged Kidney of Hypertensive 129Sv Mice
Author(s) -
Malik Zeeshan S.,
Schramm Whitney C.,
Liu Lauren P.,
Chacko Kevin M.,
Alli Abdel A.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.751.18
Subject(s) - marcks , actin cytoskeleton , cytoskeleton , kidney , actin , endocrinology , medicine , microbiology and biotechnology , downregulation and upregulation , chemistry , biology , signal transduction , protein kinase c , biochemistry , cell , gene
Essential hypertension remains a major public health problem and there is an increase in its prevalence with age. An upregulation in the expression and activity of the renal epithelial sodium channel (rENaC) plays a key role in the development of essential hypertension. MARCKS protein plays an essential role in the positive regulation of rENaC by stabilizing the actin cytoskeleton and increasing the availability of PIP2 at the apical plasma membrane. The goal of this study was to determine whether MARCKS protein expression and association with the actin cytoskeleton is augmented in the aged kidney of hypertensive 129Sv mice. 129Sv mice were aged for 12 months before being subject to salt loading (8% NaCl) for 4 weeks to induce hypertension. Blood pressure was measured by tail cuff and electrolytes in the urine were measured using an electrolyte analyzer. The kidneys were harvested for proteins and the expression of total MARCKS and phospho‐MARCKS was measured by Western blotting and densitometric analysis. Immunohistochemistry was performed to assess the localization of MARCKS and the actin cytoskeleton protein filamin A. The expression of total MARCKS protein increased while phospho‐MARCKS protein expression decreased in kidney cortex lysates from aged 129Sv mice with hypertension compared to aged 129Sv mice without hypertension. The colocalization between MARCKS and filamin A in kidney tubules was augmented in hypertensive aged mice compared to controls. These data indicate MARCKS expression and its function is augmented in the pathogenesis of hypertension in the aged kidney. Support or Funding Information This project was funded by the U.S. National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases (Grant K01 DK099617; to A.A.A.) and the University of Florida College of Medicine. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .