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Identification and Characterization of the Corticotropin‐releasing Factor (CRF) System in Atlantic Salmon ( Salmo salar )
Author(s) -
Lai Floriana,
Royan Muhammad Rahmad,
Gomes Ana Silva,
Rønnestad Ivar
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.729.2
Subject(s) - salmo , biology , gene , genome , gene duplication , genetics , evolutionary biology , in silico , subfunctionalization , chromosome , gene family , fishery , fish <actinopterygii>
In contrast to most other teleosts studied to date, salmonids has undergone four whole genome duplications (WGD), commonly referred to as the Ss4R (salmonids‐specific 4 th vertebrate WGD) event. Large genomic rearrangements and the restoring of the disomic chromosome inheritance in the ancient polyploidy have been crucial for the post Ss4R re‐diplodization process, leading to possible divergent functions of duplicated genes. This evolutionary genome duplication, together with the available genome sequence information for Atlantic salmon ( Salmo salar ), provide a unique opportunity to investigate the pivotal roles of gene diversity and functionality. When facing variable external or internal adverse effects beyond their homeostatic state, teleosts engage a stress response through the Hypothalamic‐Pituitary–Interrenal gland (HPI) axis to restore the compromised physiological steady state and enable the animal to overcome the threat. Corticotropin‐releasing factor (CRF), CRF‐binding proteins (CRFBPs) and CRF receptors (CRFRs) are commonly used as markers for stress responsiveness. This system has been poorly explored in Atlantic salmon, thus, in this study, we aim to investigate all the CRF system players. As for other salmonids, the Ss4R event induced the tetradiploidization in the Atlantic salmon genome leading to the several duplicated genes (paralogues) in these species. We currently investigate the crf , crfbp and crfr genes to explore the roles of the different paralogues. In silico analysis show that the Atlantic salmon genome has four crf paralogue genes distributed among chromosome ( Chr)Ssa03 , ChrSsa14, ChrSsa19 and ChrSsa 29, two crfbp genes located in the ChrSsa01 and ChrSs11 , two crf r1 paralogue genes placed in the ChrSsa03 and ChrSsa06 and two crfr2 paralogue genes in ChrSsa03 and ChrSsa14 . To evaluate their function in the HPI stress response axis, we have analysed the mRNA expression of the different transcripts in different brain regions of Atlantic salmon post‐smolts. Our initial data shows that all crf transcripts are present in the hypothalamus and pituitary. However, further analysis are needed and particularly the characterization of these several transcripts under control and stressed conditions. Support or Funding Information Funded by the Research Council of Norway (NFR 267626 LeuSENSE). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .