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The increased expression of microRNAs 451, 638 and 362 in Urinary Exosomes of Human Subjects profiled as Diabetic and Hypertensive
Author(s) -
Lewis Harvey,
Smith Michael,
Fluitt Maurice,
Khundmiri Syed J,
Ecelbarger Carolyn,
Lee Dexter L
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.716.5
Subject(s) - medicine , diabetes mellitus , diabetic nephropathy , endocrinology , urinary system , urine , urology
Urinary Exosomes (UE) are involved in several metabolic processes, including hypertension and are considered to be vehicles for eradicating cell waste products. UE have important functions in human diseases as carriers of information including, proteins, lipids and miRNA (mi‐R). Because of their high stability, UE mi‐Rs can be useful predictors of the progression of diabetes and hypertension. UE mi‐Rs 451, 638, 362 and 16 are potential biomarkers for early diabetic nephropathy, salt‐sensitive hypertension and cardiovascular disease, respectively. We hypothesized that mi‐Rs 451, 638, 362 and 16 are increased in diabetic + hypertensive patients when compared to controls. To address this hypothesis, human urine samples were collected from control, diabetic and hypertensive patients. UE were isolated in each group and mi‐R 451, 638, 362 and 16 expression was quantified by RT‐PCR. mi‐R expression was expressed as log2, normalized to U6 small nuclear RNA as mean and standard error. UE mi‐R 451 expression in diabetic + hypertensive patients was 2.4 ±1.8 and 0.5 ±0.8 in controls. UE mi‐R 638 expression in diabetic + hypertensive patients was 1.0 ±2.0 and 0.1 ±1.7 in controls. UE mi‐R 362 expression in diabetic + hypertensive patients was 1.9 ±1.8 and 0.2 ±0.8 in controls. UE mi‐R 16 expression in diabetic + hypertensive patients was similar to the controls (0.1 ±10 and −9 ±1, respectively). Our results suggest that both mi‐R 451, 638 and 362 expressions are increased in diabetic + hypertensive patients. There was not an increase in UE mi‐R 16 in diabetic + hypertensive patients when compared to controls. In conclusion, mi‐R 451, 638 and 362 expressions may serve as non‐invasive biomarkers for the early detection for diabetes and hypertension. Support or Funding Information R25AG047843‐NIH This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .