Premium
Acute exposure to high‐induction electromagnetic field affects activity of model peripheral sensory neurons
Author(s) -
Dittert Ivan,
Prucha Jaroslav,
Krusek Jan,
Sinica Viktor,
Kadkova Anna,
Vlachova Viktorie
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.706.4
Subject(s) - sensory system , neuroscience , dorsal root ganglion , sensory neuron , stimulation , calcium imaging , neuropeptide , transient receptor potential channel , calcium , electrophysiology , voltage dependent calcium channel , receptor , biology , medicine
Exposure to repetitive low‐frequency electromagnetic field (LF‐EMF) shows promise as a non‐invasive approach to treat various sensory and neurological disorders. Despite considerable progress in the development of modern stimulation devices, there is a limited understanding of the mechanisms underlying their biological effects and potential targets at the cellular level. A significant impact of electromagnetic field on voltage‐gated calcium channels and downstream signaling pathways has been convincingly demonstrated in many distinct cell types. However, evidence for clear effects on primary sensory neurons that particularly may be responsible for the analgesic actions of LF‐EMF is still lacking. Here, we used F11 cells derived from a fusion between mouse embryonic neuroblastoma and rat dorsal root ganglion (DRG) neurons as an authentic in vitro model of peripheral sensory neurons. These cells retain many features of native DRG neurons, including expression of neuropeptide receptors and various voltage‐gated channels (Vetter a Lewis, 2010, Yin et al., 2016). Using calcium‐imaging, we examined the effects of three different protocols of high induction electromagnetic stimulation on F11 chemical responsiveness and spontaneous activity. We show that short‐term (< 180 s) exposure to LF‐EMF reduces calcium transients in response to bradykinin, a potent pain‐producing inflammatory agent formed at sites of injury. Moreover, we characterize an immediate and reversible potentiating effect of LF‐EMF on neuronal spontaneous activity. Our results provide new evidence that electromagnetic field may directly modulate the activity of sensory neurons and highlight the potential of sensory neuron‐derived cell line as a physiologically relevant tool for studying the effects of LF‐EMF, and the underlying mechanisms at the cellular and molecular level. Support or Funding Information This work was supported by the Ministry of Health of the Czech Republic (NV16‐28784A) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .