Premium
Biostimulating Effect of 685 nm Low‐Intensity Laser Radiation on Cell Viability in Multicellular Spheroids Culture
Author(s) -
Gastaldi Gabriela Gomes Cardoso,
Oliveira Juliana Paula,
Andréa Dernowsek Janaína,
Rezende Rodrigo Alvarenga,
Silva Jorge Vicente Lopes,
Parizotto Nivaldo Antônio,
Amaral Creusa Sayuri Tahara,
Amaral André Capaldo
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.705.8
Subject(s) - viability assay , irradiation , agarose , cell culture , in vivo , biophysics , spheroid , in vitro , cell , chemistry , fetal bovine serum , andrology , microbiology and biotechnology , biology , biochemistry , medicine , physics , genetics , nuclear physics
Recent research evidences substantial morphological and physiological differences between cells maintained in vitro in two‐dimensional (2D) and three‐dimensional (3D) culture conditions. The current consensus is that 3D culture is better because it precisely mimics the cellular microenvironment in vivo . The biomodulatory effect of low‐intensity laser radiation (LILR) on cells in 2D cultures is already well established. On the other hand, the effect of this radiation on cells in 3D cultures has not yet been widely investigated. The aim of this work was to analyze the biomodulatory effect of LILR, at the wavelength (ƛ) of 685 nm, on the viability of osteogenic precursor cells (OPC) cultured as multicellular spheroids (MS). Agarose molds containning wells (300 μm diameter) were used for MS formation using the non‐adherent surface culture concept. A cell count (2×10 5 ) of the OPC lineage (MC3T3) was seeded in each mold and maintained under ideal growth conditions (Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum/37°C/95% humidified atmospheric air/5% CO 2 ). The molds were irradiated for 5 consecutive days at doses of 0.5, 1.0 or 1.5 J/cm 2 (triplicates), and the first irradiation was performed immediately after seeding. After this period, the cultures were submitted to cell viability assay and the results were expressed as a percentage of non‐irradiated control cultures (ANOVA ONE‐WAY, p≤0.05). The results showed that LILR, at ƛ 685 nm, provided a 79% increase in cell viability at the dose of 1J/cm 2 . The remaining doses exhibited similar viability rates that the control. These results confirm the dose‐dependent biostimulatory effect of LILR at ƛ 685 nm on MS culture and contribute to the understanding of the real influence of this radiation on cells under ideal conditions for in vitro simulation studies. Support or Funding Information Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ‐ CAPES ‐ Brazil This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .