Premium
Effects of differential expression of cytokines secreted by rhesus monkeys bone marrow mesenchymal stromal cells on age‐related bone aging and autophagy
Author(s) -
Liu Fei,
Li Yuanmin,
Zhang Jie,
Lu Yanrong,
Chen Younan,
Cheng Jingqiu
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.705.7
Subject(s) - mesenchymal stem cell , transcriptome , telomerase , stromal cell , autophagy , senescence , microbiology and biotechnology , bone marrow , kegg , biology , stem cell , programmed cell death , gene expression , gene , immunology , cancer research , apoptosis , genetics
Background Aging is a gradual process characterized by a deterioration in the function of the organism. Studies have shown that there may be a close relationship between organ/tissue aging and stem cells. In addition, progressive DNA damage and mitochondrial dysfunction are considered to be major causes of natural aging. Moreover, autophagy plays an important role in maintaining skeletal homeostasis, and changes in this pathway are associated with osteoporosis. Therefore, the search for age‐related bone aging mechanisms can explain the progressive decline of organs and the development of diseases associated with advanced age, and can clarify the ways in which the relevant therapeutic methods are developed. Methods We isolated bone marrow mesenchymal stem cells (rBMSCs) from aged, middle‐aged, and young rhesus monkeys and cultured them to passage 3, followed by transcriptome sequencing of three different age‐derived cells to explore aging‐related GO term and KEGG Pathway. Further analysis of hub genes were performed by gene co‐expression network analysis. In addition, we also measured telomerase activity, cell death and β‐galactosidase staining in rBMSCs of different ages. Results We successfully isolated and cultivated old, middle and young rBMSCs. As shown in Figure 1D, aged rBMSCs stained the most prominent positive galactosidase, while Figure 1C showed that the telomerase activity of rBMSCS gradually decreased from young to old, and the same results also be obtained by cell death analysis and cell culture supernatants (Figure 1A, B). In addition, we found the enrichment of aging‐related genes through both GO term and KEGG pathway analysis (Figure 2). Eleven hub genes were further obtained by the protein interaction map, including LRRC17, VILL, PENK, IFIT1B, MX1, PTGFR, GJB2, IFI44L, CHI3L, LOC718478, GBP2 (Figure 3). Among them, LRRC17 has been reported to participate in and maintain bone homeostasis of osteoblasts and osteoclasts. Conclusions We initially explored the effects of age on rBMSCs and possible hub genes in the aging process. The aging characterization of rBMSCs in different age groups all showed a significant decreasing trend. Eleven key factors, including LRRC17, may be involved in the process of bone aging. Taken together, these results suggest several new key factor in bone aging and related aging pathways. Support or Funding Information This work was supported by the Chengdu Giant Panda Breeding Research Foundation (CPF2015‐08). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .