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Histological Analysis of Lysophosphatidic Acid Receptor 3 Deficient Zebrafish
Author(s) -
Chen WeiMin,
Chang YaChi,
Lin YuNung,
Chiang JuiChung,
Lee Hsinyu
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.705.5
Subject(s) - lysophosphatidic acid , zebrafish , biology , erythropoiesis , receptor , microbiology and biotechnology , pathology , medicine , gene , biochemistry , anemia
Lysophosphatidic acid (LPA) is a small bioactive phospholipid enriched in serum and exerts diverse cellular functions through activation of multiple G Protein‐coupled receptors, LPA 1 ‐LPA 6 . Our previous studies demonstrated that lysophosphatidic acid receptor 3 (LPA 3 ) is critical for megakaryopoiesis and erythropoiesis in zebrafish. However, the roles of LPA 3 during the development of other organs of zebrafish remain unclear. Here, we established a zebrafish strain with targeted disruption of lysophosphatidic acid receptor 3 ( Lpar3 ) gene by Transcription Activator‐like Effector Nuclease (TALEN). Paraffin‐section and hematoxylin‐eosin staining were performed for histological analysis to clarify the effects of LPA 3 in different organs of adult zebrafish. Histological analysis results showed a shrinking of hematopoietic tissue in kidney marrow of LPA 3 deficient fish, implies essential roles of LPA 3 in hematopoiesis. In addition, bile duct hyperplasia and mature spermatids loss were also observed in LPA 3 deficient fish. Nevertheless, no obvious morphological differences in eyes, thymus, heart, and spleen were observed. Our observation provides tissues profiles of LPA 3 −/− animal and possible application of LPA 3 deficient zebrafish as a disease model in the future. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .