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Oxidative stress in concurrent hypercaloric diet and caffeine consumption in Wistar rats: Possible modulatory effects of aqueous extract of curcuma longa
Author(s) -
Azeez Odunayo I.,
Adeshina Wuraola A.,
Oyagbemi Ademola A.,
Ajibade Temitayo O.,
Omobowale Temitayo O.,
Nabofa Williams E.
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.694.15
Subject(s) - curcumin , medicine , curcuma , oxidative stress , caffeine , obesity , calorie , endocrinology , diabetes mellitus , physiology , traditional medicine , pharmacology
Obesity and its complications have become a menace, not only in the developed world, but in developing countries as a result of sedentary lifestyles, consumption of high caloric diets and lack of exercise. This has therefore increased the incidence of complications of obesity such as diabetes, hypertension and stroke. The current study was designed to evaluate the effects of high calorie diet and caffeine consumption as well as the modulatory effects of aqueous extract of Curcuma longa in the Wistar rats. Forty‐eight 3 weeks old male Wistar rats were used for the study. The rats were divided into eight groups of six rats each. Groups A – C were given normal rat chow while groups D – H were given high calorie diet, for 14 weeks. At fourteen weeks Group B and E were administered caffeine (5 mg/kg), Group C, Curcumin extract (500 mg/kg) while Group A and D were given water only. Group F received caffeine and curcumin extract, Group G, curcumin only while Group H received vitamin C. All the treatment was carried out for 28 days, while each of them were receiving their respective normal and high calorie diets. Blood samples were collected at 2 and 4 weeks of treatment for haematology and erythrocyte osmotic fragility. Oral glucose tolerance test (OGTT) and blood pressure were also determined while liver and kidney samples were collected for determination of markers of oxidative stress. High calorie diet caused oxidative stress in the affected animals and predisposed to insulin resistance due to failure of blood glucose to reduce during OGTT. It also caused anaemia, hypotension and decreased erythrocyte osmotic resistance. Caffeine consumption on the other hand showed beneficial effects especially in the blockage of reactive oxygen species and other free radicals, lipid peroxidation, thereby reducing oxidative stress and liver and kidney damages. Aqueous extract of Curcuma longa however did not show appreciable antioxidant potential due to its lack of phenolic compounds and flavonoids. It however contains alkaloids, tannins, curcumin and terpenoids, which may have other benefits such as anti‐inflammatory actions. Support or Funding Information None This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .