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Obesogenic diet in aging mice disrupts gut microbe composition and alters neutrophil:lymphocyte ratio leading to inflamed milieu in acute heart failure
Author(s) -
Kain Vasundhara,
Van Der Pol William,
Mariappan Nithya,
Ahmad Aftab,
Eipers Peter,
Morrow Casey,
Gibson Deanna L,
Gladine Cecile,
Vigor Claire,
Durand Thierry,
Halade Ganesh V
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.693.6
Subject(s) - gut flora , immunology , immune system , inflammation , biology , systemic inflammation , physiology , medicine
Calorie dense obesogenic diet (OBD) is a prime risk factor for cardiovascular disease in aging. However, increasing age coupled with changes in the diet can affect the interaction of intestinal microbiota impacting immune system that can lead to chronic inflammation. How the age and calorie enriched OBD interact with microbial flora and impacts leukocyte profiling is currently under‐investigated. Here, we tested whether OBD in young and aging mice alters the gut microbe composition and the splenic leukocyte profile in acute heart failure. Young (2 month‐old) and aging (18 month‐old) mice were supplemented with STD‐(standard chow diet ~4% safflower oil diet) and OBD‐(obesogenic diet‐10% safflower oil) diet for 2 months and then subjected to coronary artery ligation to induce myocardial infarction (MI). Fecal samples were collected pre‐ and post‐diet and the microbial flora analyzed using 16S V4 rRNA gene DNA sequencing and QIIME informatics. The STD and OBD in aging mice resulted in an expansion of the genus Allobaculum in the fecal microbiota. However, we found a pathological change of neutrophil: lymphocyte ratio in aging mice in comparison to their young counterparts. Thus, calorie‐enriched OBD dysregulated splenic leukocytes by decreasing immune responsive F4/80+/CD169+ macrophages in aging. OBD programmed neutrophil swarming with an increase in isoprostanes levels with dysregulation of lipoxygenases and cytokines. In summary, calorie dense OBD in aging mice disrupted the composition of the gut microbiota that correlates with the development of integrative and system‐wide non‐resolving inflammation in acute heart failure. Support or Funding Information Authors acknowledge the support from National Institutes of Health (NIH)‐NCCIH (formerly known as NCCAM) AT006704, HL132989‐01 to GVH and American Heart Association postdoctoral fellowship [POST318] to V. K. The authors also acknowledge support of Microbiome Resource at the University of Alabama at Birmingham: Comprehensive Cancer Center (P30AR050948), Center for Clinical Translational Science (UL1TR001417), University‐Wide Institutional Core, Heflin Center for Genomic Sciences and Microbiome Center to CM This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .