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Role of HMGB1 Protein in Experimental Myocardial Infarction
Author(s) -
Cebova Martina,
Barta Andrej,
Kosutova Michaela,
Pechanova Olga
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.690.2
Subject(s) - hmgb1 , myocardial infarction , medicine , ligation , cardiology , western blot , nitric oxide , nitric oxide synthase , aorta , infarction , chemistry , inflammation , biochemistry , gene
Myocardial infarction (MI) results from a reduction in coronary blood flow which is extensive enough to render the oxygen supply to myocardial tissues insufficient. MI remains a leading cause of morbidity and mortality among all cardiovascular diseases over the world. High mobility group box 1 (HMGB1) is a non‐histone chromosomal protein with multiple cardioprotective effects. The aim of the study was to evaluate the effects of HMGB1 protein on biochemical and morphological parameters after experimental MI. Methods 12‐week‐old male WKY rats used for the study were divided into following groups: shame operated WKY without MI, WKY with MI, WKY + IM+ anti‐HMGB1 protein. In vivo model of experimental MI was induced by ligation of the left descending coronary artery and lasted 20 min. Before reperfusion anti‐HMGB1 protein was administrated i.v. Animals survived 7 days after MI. Nitric oxide synthase (NOS) activity was determined by conversion of 3 [H] Arginine to 3 [H] Citrulline in the aorta and ischemic, border and non‐ischemic region of the heart. NFκB expression was determined by Western blot. For morphological parameters, the hearts were excised and used for TTC‐staining procedure. Cytokine levels were investigated using the Bio‐Plex Pro Cytokine kit in the plasma. Concentration of CD was measured spectrophotometrically. Results Anti‐HMGB1 protein increased NOS activity in both ischemic and border part of the heart, as well as in the aorta, on the other hand NOS activity did not change in non‐ischemic part of the heart. The protein administration significantly decreased NFκB expression in MI part of the heart, as well as TNF‐alpha and IL‐6 level in plasma. Simultaneously, anti HMGB1 protein decreased MI part as well as border region of the heart. Discussion Considering the results, HMGB1 protein is a promising molecule for reduction the negative effects of the myocardium infarction, as well as a promising agent for the treatment of cardiovascular diseases. Support or Funding Information Supported by: VEGA 2/0170/17, APVV‐14‐0932 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .