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PARTICIPATION OF ESTRADIOL AND NITRIC OXIDE ON THE VASOCONSTRICTOR EFFECT OF BRADIKYNIN
Author(s) -
Campos Maria Elena Hernández,
Romero Karla Stephania Dominguez,
Hernandez Ignacio Valencia
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.677.3
Subject(s) - bradykinin , ovariectomized rat , medicine , endocrinology , nitric oxide , vasodilation , vasoconstriction , estrogen , angiotensin ii , angiotensin converting enzyme , chemistry , endothelium , hormone , nitric oxide synthase , blood pressure , receptor
Ovarian hormones privation in menopause can contribute to development of hypertension, which has high morbimortality in the world. Estrogens protect cardiovascular system by reducing angiotensin‐converting enzyme (ACE) activity, preventing bradykinin degradation and promoting a reduction in the vascular tone. These actions of estrogen are related with an increase in the synthesis of nitric oxide. However, bradykinin can produce, under certain conditions, vasoconstriction, a much less studied effect. Therefore, the objective of this work was to study the role of 17‐β estradiol (E 2 ) on the vasoconstrictor effect of bradykinin. We used a group of 24 Wistar ovariectomized rats (OVX) and 6 Sham rats. After 14 days of recovery, a group of 6 OVX rats were administered wit E 2 (5 μg/Kg/day s.c. for 5 days) and 6 OVX rats were administered with corn oil (vehicle). At the end of the treatment, rats were sacrificed and thoracic aorta extracted and cut in 5 mm length rings, subsets of aortas were obtained with and others without endothelium. Bradikynin concentration‐response curves were constructed, both in the presence and absence of L‐NAME. All procedures in animals complied with NOM‐062‐ZOO‐1999 and institutional animal care committee. The results showed that ovariectomy or treatment with estradiol did not modify blood pressure values. Treatment with estradiol, however, significantly reduced heart rate in OVX rats. Bradykinin produced a concentration‐dependent contractile effect that was of greater magnitude in rings without endothelium and was blocked by HOE140. Incubation with L‐NAME did not modify the contractile effect of bradykinin in the aortic rings from SHAM rats but inhibited this effect in the OVX treated rat with E 2 . These results indicate that in the presence of ovarian hormones, where estradiol seems to play an important role, bradykinin produces contraction independently of the endothelium and NO, this contraction seems to be mediated by the activation of B 2 receptors. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .