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Oxidative Stability and Antiproliferative Effect of Fish Oil Commercial Supplements on Cultured Human Keratinocytes
Author(s) -
Oliveira Carvalho Patricia,
Santis Gabrielle Kristine Doratiotto,
Franco Yollanda Edwirges Moreira,
Barba Fatima Cristine Ribeiro,
Souza dos Santos Gisele Karine,
Longato Giovanna Barbarini
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.672.8
Subject(s) - hacat , polyunsaturated fatty acid , fish oil , chemistry , oxidative phosphorylation , biochemistry , food science , in vitro , fatty acid , fish <actinopterygii> , biology , fishery
Fish oil, due high concentration of n‐3 polyunsaturated fatty acids (n‐3 PUFA), reduce symptoms in many inflammatory skin diseases, limiting the spreading of the inflammatory process. Oxidized derivative was shown to play an important role in the antiproliferative properties of n‐3 PUFA. The objective of this study was to assess the PUFA content, oxidative stability and antiproliferative effects on cultured human keratinocytes of fish oil commercial supplements. Lipid oxidation was measured by colorimetric reactions and the fatty acid composition was measured using gas chromatography. Primary (peroxide), secondary (anisidine), and total oxidation products levels of two supplements exceeded maximum established by international standards of quality. In vitro inhibitory activity against HaCat cell lines, measured with the tetrazolium MTT (3‐(4,5‐dimethylthiazolyl‐2)‐2,5‐diphenyltetrazolium bromide) assay, showed that the supplement of higher oxidation content exerted the most potent antiproliferative effect (IG 50 86.54 μg/mL). Our data provide additional information regarding the preferential antiproliferative effect of oxidized derivatives of n‐3 PUFA in cultured human keratinocytes Support or Funding Information FAPESP and CAPES (Finance Code 001) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .