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Establishment of Optimum Conditions for Antioxidant Components Containing Curcuma L Longa (Turmeric) Leaf Extraction using Response Surface Methodology
Author(s) -
Kim Sera,
Ko SeokChun,
Kim YoonSook,
Ha SangKeun,
Park HoYoung,
Park YongGon,
Lee SangHoon
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.672.4
Subject(s) - curcuma , extraction (chemistry) , response surface methodology , chemistry , abts , dpph , antioxidant , traditional medicine , central composite design , curcumin , chromatography , food science , biochemistry , medicine
Curcuma L longa (turmeric) is a functional material but its leaves are wasted as byproduct. This study focused on the possibility of turmeric leaves as a functional food material by establishing its extraction condition using a reflux condenser with water and different concentrations of ethanol. According to the results, water extraction was efficient for extracting its total phenolic compounds (2.741±0.099 μ g/ml), flavonoids (4.776±0.010 μ g/ml) within various temperature and time conditions. DPPH (486.78±12.73 μ g/ml), ABTS (123.19±2.42 μ g/ml), and H 2 O 2 (849.06±23.07 μ g/ml) radical scavenging activities were also high in water extraction. We used response surface methodology (RSM) to optimize extraction conditions for Curcuma L longa leaves. After establishing the extraction ratio, we applied a central composite design to identify the effects of independent variables, temperature (X 1 ), time (X 2 ) to dependent variables, yield (Y 1 ). From these results, the optimal conditions were established as 150 minutes with a 1:25 ratio at 85°C, giving a 15.57% extraction yield. Using the leaf extract, we measured cell viability and cytokine production using Raw 264.7 cell line to confirm its anti‐inflammatory effects. We confirmed there is no cytotoxicity in Curcuma L longa leaf extract treat groups compared to lipopolysaccharide treat (control) group. Also, production of cytokines such as TNF‐α, MCP‐1 and IL‐1β in Curcuma L longa leaf extract treat groups , decreased comparing with control group. In this study, we confirmed the optimum extract conditions of turmeric leaves as fundamental data for its application in the industry. Furthermore, we provide a new insight on turmeric leaf extract as a health‐beneficial natural product by identifying its antioxidant and anti‐inflammatory properties. Support or Funding Information This work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through High Value‐added Food Technology Development Program, funded by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) (117078‐03). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .