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The Effects of Serotonin‐2A Receptor Modulation on Glutamate Levels in the Medial Prefrontal Cortex following Methamphetamine Self‐Administration
Author(s) -
Dolezal Alexandra,
McFadden Lisa,
Severson Marie
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.664.13
Subject(s) - meth , methamphetamine , prefrontal cortex , glutamate receptor , 5 ht receptor , microdialysis , self administration , pharmacology , psychosis , serotonin , neuroscience , medicine , psychology , receptor , chemistry , dopamine , psychiatry , cognition , monomer , organic chemistry , acrylate , polymer
Methamphetamine (METH) use can lead to psychosis‐like behaviors. Previous studies have indicated that there is an increase in frontal cortex serotonin (5HT)‐2A receptors as well as observed hallucinogenic‐drug induced behaviors following METH self‐administration. The 5HT‐2A receptor is thought to play a role not only in psychosis‐like behaviors, but also the regulation of glutamate in the frontal cortex. Our research seeks to determine if METH self‐administration will lead to alterations in METH ‐induced glutamate release in the frontal cortex. Further, the role of the 5HT‐2A receptor in these changes will be explored. Male Sprague‐Dawley rats received METH or saline self‐administration 8 hours/day for 7 days. Two days following self‐administration, a microdialysis probe was inserted into the medial prefrontal cortex. Following basal assessment, animals received an injection of the 5HT‐2A antagonist or vehicle followed by a 5 mg/kg injection of METH or saline. Basal and METH ‐induced glutamate levels were assessed. Preliminary results suggest that prior METH self‐administration result in higher glutamate levels in the medial prefrontal cortex following a vehicle injection and a METH challenge. Ongoing efforts will determine if pretreatment with the 5HT‐2A antagonist will reduce these changes in glutamate. Overall, the results of this study will determine the role of the 5HT‐2A receptor in regulating glutamatergic activity in the frontal cortex following METH self‐administration. Support or Funding Information This research was supported by the National Institute on Drug Abuse of the National Institutes of Health (DA036012) and provided by the NIDA grant (R25‐DA033674). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .