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Circulating exosomes of long non‐coding RNA DANCR correlated to prognosis of Hepatitis C virus‐related hepatocellular carcinoma
Author(s) -
Wang ShuChi,
Tu WenYu,
Hsieh MengHsuan,
Huang JeeFu,
Chuang WanLong,
Dai ChiaYen,
Yu MingLung
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.662.69
Subject(s) - hepatocellular carcinoma , microvesicles , hepatitis c virus , medicine , exosome , cancer research , cancer , oncology , virus , immunology , biology , microrna , gene , biochemistry
Hepatitis C virus (HCV) is one of major etiologies of hepatocellular carcinoma (HCC) worldwide. HCC is a highly prevalent and deadly cancer because of high risks of recurrence and/or metastasis. Identification of potential prognostic markers and/or target molecules could help in the preventing and managing HCV‐related HCC after the success of DAA treatment. Extracellular exosomes contain numerous signaling molecules including lncRNAs and play an important role in intercellular communication. To develop of liquid biopsy for prognostic markers of Hepatitis C Virus‐related hepatocellular carcinoma after surgical resection, we determined DANCR expression level in circulating exosomes. Total exosomes were extracted from 200 μL of serum by exosome precipitation solution and quantification DANCR by qPCR analysis. Results indicates exosomes DANCR levels was significantly higher among HCV‐related HCC patients with post‐resection recurrence than those without. Higher versus low exosomes DANCR with cut‐off at −7.5dCt [lncDANCR‐U6]) could predict HCV‐HCC recurrence (area under curve: 0.83). The 5‐y cumulative incidence of recurrence and mortality was 90.0% and 37.7%, respectively, among patients with higher exosomes DANCR, compared to 39.5% and 20.6%, respectively, among those with lower exosomes DANCR with harzard ratio (CI) of 3.8 (2.52–5.84) and 2.2 (1.29–3.89), respectively, by Cox regression model. The diagnostic power for HCC recurrence among HCV‐HCC patients was better with exosomes DANCR than with hepatic tumor DANCR or Tumor/Non‐tumor DANCR ratio. By knockdown or overexpression of DANCR in JFH lo (long‐term HCV‐infectious) cell models, we observed that increased DANCR enhanced cell migration and invasion ability by regulating mesenchymal‐associated β‐catenin expression. In this study, we demonstrated that circulating exosomes lnc‐DANCR could serve as a liquid biopsy for prognostic biomarker of HCC recurrence after surgical resection for HCV‐HCC. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .