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Molecular and histopathological diagnosis of Fowlpox virus in Chickens in Nigeria
Author(s) -
Adesina Rofiat,
Ogunleye Seto,
Alaka Olugbenga,
Fagbohun Olusegun
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.662.60
Subject(s) - fowlpox virus , histopathology , pathology , biology , polymerase chain reaction , virology , medicine , gene , recombinant dna , biochemistry
Fowlpox is a relatively slow developing disease characterized by discrete, proliferative lesions on the skin of toes, legs or head, and/or mucous membranes of the mouth and upper respiratory tract of birds. Several diseases of birds such as infectious coryza, infectious laryngotracheitis, Mareks, and vitamin A deficiency can be confused with fowlpox, therefore there is need for an accurate and reliable method of diagnosis. Polymerase Chain Reaction (PCR) and histopathology can be used to arrive at the confirmatory diagnosis of the disease. Consequently, fifty samples from suspected cases of fowlpox from backyard poultry in Ibadan, Nigeria were collected, and PCR was used to detect and characterize the open reading frame 168 (39 kDa core protein) gene of the virus. Also, lesions from the eyelids excised were and histopathology was carried out. Thirty‐one out of 50 samples (62%) tested positive for FPV. Histopathology revealed the presence of dermal hyperplasia and Bollinger bodies which are confirmatory of fowlpox. PCR amplification of 39 kDa core protein gene and histopathology provide a reliable diagnosis of fowlpox. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .