Premium
Natural Rubber Latex as Stem Cell Scaffold for Muscle Regeneration
Author(s) -
Floriano Juliana Ferreira,
Oliveira Graeff Carlos Frederico,
Barbosa Angélica Mércia Pascon,
Marcondes João Paulo,
Pinto Taís Silva,
Zambuzzi Willian Fernando,
Rudge Marilza Vieira Cunha
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.662.18
Subject(s) - hevea brasiliensis , materials science , scaffold , biomaterial , biomedical engineering , natural rubber , stem cell , biocompatibility , chemistry , microbiology and biotechnology , nanotechnology , biology , composite material , medicine , metallurgy
Natural rubber latex (NRL) is a product extracted from rubber trees. The most common source of NRL is Hevea brasiliensis , NRL is a mixing colloidal rubber particle stabilized by a thin layer of phospholipids and proteins. The NRL has several features that make it an excellent scaffold and biomaterial to promote the growth and repair of tissues, due to their characteristics, like biocompatibility, angiogenic potential, flexibility, mechanical stability, surface porosity, permeability and low cost. The present study aimed at assessing the potential NRL use as stem cell scaffold, for the future use in muscle regeneration and cell therapy. The NRL membranes were produced with latex collected from RRIM 600 clone, the NRL was stored at a low temperature to prevent spontaneous coagulation and was centrifuged for 5 min at 1,500 rpm to remove impurities gathered in the collection process. The membranes were prepared by depositing a thin layer of NRL covering the bottom of a polystyrene petri dish measuring 60 mm in diameter and carried to a oven, where it was dried at 50 °C. The study was approved by Botucatu Medical School ethics committee on the use of animals (CEUA number 1234/2017). The mesenchymal stem cells were isolated from the bone marrow of the femur of young rats and cultured until the fourth passage. After which the cells were characterized by immunocytochemistry and immunophenotyping techniques, cell adhesion on the surface of the NRL membrane after 7 days of sowing the cells was assessed by scanning electron microscopy (SEM), confocal microscopy, zymography techniques for the evaluation of metalloproteinases 2 and 9, electrophoresis and protein expression for integrins. Our partial results showed that 98% of the cells expressed CD90, besides adhesion to plastic and the ability to differentiate and formation of fibroblast colonies, according to the methods indicated by the International Society for Stem Cell. After being seeding the surface of the NRL membranes it was verified high cell viability, excellent cell adhesion high cell proliferation rate, demonstrating the great potential NRL as stem cell scaffold. Support or Funding Information This project is funded by São Paulo Research Foundation – FAPESP (number grant 2016/01743‐5) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .