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A Transcriptomics and Functional Genetics Approach to Identifying Components of the Drosophila Polyamine Transport System
Author(s) -
Saldanha Joanne,
Haney Michael,
Landry Brianne,
Figueroa Natalia,
Kalm Laurence
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.652.18
Subject(s) - polyamine , transcriptome , biology , gene , spermidine , microbiology and biotechnology , computational biology , biochemistry , gene expression , enzyme
Polyamines are essential growth factors for normal and malignant cells. Cells obtain polyamines via biosynthesis or transport from the extracellular environment. Polyamine depletion therapy is an attractive option to inhibit the growth of cancer cells. An FDA approved drug inhibits polyamine biosynthesis, however malignant cells typically respond by upregulating the polyamine transport system. Therefore, a dual therapy simultaneously targeting the biosynthetic and transport pathways is desirable. However, this approach is currently hampered by a poor understanding of the transport system in multicellular eukaryotes. To address this problem, we used RNA‐Seq to identify genes that are up‐ or down‐regulated under conditions where Drosophila larvae must transport polyamines to survive. From the screen 36 candidate genes for roles in polyamine transport were identified. We have also developed a functional genetic assay to test individual candidate genes. Our results suggest the existence of tissue‐specific polyamine transport systems, and post‐transcriptional regulation of polyamine transport. Support or Funding Information University of Central Florida, College of Sciences Seed Award This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .