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Microbial VOC Fingerprints: Rapid Detection of Antimicrobial Resistance in Pathogenic Bacteria
Author(s) -
Dailey Allyson,
Saha Jessica,
Zaidi Fatima,
Couch Robin
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.639.3
Subject(s) - antimicrobial , francisella tularensis , antibiotic resistance , bacteria , microbiology and biotechnology , isolation (microbiology) , yersinia pestis , biology , chemistry , antibiotics , biochemistry , genetics , virulence , gene
The US Centers for Disease Control and Prevention (CDC) estimates that at minimum, 2 million people in the United States are infected with antimicrobial resistant (AMR) bacteria, resulting in 23,000 deaths each year. With no projected decrease in the number of deaths and the evergrowing threat of multi‐drug resistant bacteria, the development of a rapid and accurate diagnostic test is crucial, not only to reduce the burden on the public healthcare system, but to save countless lives. Microbial volatile organic compounds (mVOCs) are a structurally diverse group of microbial‐derived metabolites, generally related by their volatility at ambient temperature. There is great diagnostic potential for mVOCs, as numerous studies have identified unique mVOC‐fingerprints indicative of specific bacterial pathogens. In addition, specialized headspace sampling methods, such as solid‐phase microextraction (hSPME), coupled to gas chromatography, have enhanced the isolation, preconcentration, and analysis of mVOCs from biological specimens. Herein, we describe the development and assessment of our newly developed and patented metabolomics device and technique known as “simultaneous multifiber headspace solid‐phase microextraction (simulti‐hSPME),” for the rapid and minimally invasive preparation of mVOC fingerprints. This method is evaluated against antimicrobial susceptible and resistant strains of Yersinia pestis and Francisella tularensis . Support or Funding Information Funding for this project is provided by DTRA (Grant Number: HDTRA 1‐16‐1‐0040). This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .